With NaveniFlex you can detect protein-protein interactions, post-translational modifications, for example phosphorylation, localize endogenous proteins with higher specificty and study receptor activation with the possibility of relative quantification. All NaveniFlex products are based on secondary detection by Navenibodies (carefully selected seconday antibodies conjugated to proprietary oligo arms). The Navenibodies enable a highly sensitive and specific readout of the selected target.
Applications of NaveniFlex proximity ligation technology
Choose kit by application
| Application | NaveniFlex MR | NaveniFlex MM | NaveniFlex RR |
|---|---|---|---|
| Protein interactions | |||
| Protein modifications | |||
| Protein expression |
Biomarker targets
NaveniFlex MR
Protein interaction: Chromatin interactions (histone H3) with the nuclear lamina (lamin-B1).
Cell line: BT474 (breast cancer cells)
Detection fluorophore: TEX615
Protein interaction of beta-catenin and e-cadherin
Tissue Human skin tissue
Detection fluorophore: ATTO488
Protein interaction of beta-catenin and e-cadherin
Cells MCF7 cells
Detection fluorophore: Atto647N
Protein detection HER2, protein detection
Cells BT474 (breast cancer cells)
Detection fluorophore: TEX615
NaveniFlex MM
Protein detection CA125
Tissue: CA-125, 5 + Control Slide, Sigma Aldrich
Detection fluorophore: TEX615
Protein detection Histone H3
Cells: BT474 (breast cancer cells)
Detection fluorophore: TEX615
Protein detection Histone H3
Tissue: Human skin
Detection fluorophore: TEX615
NaveniFlex RR
Protein detection PDL1
Cells: HDLM-2, SignalSlide® PD-L1 IHC Controls 13747
Detection fluorophore: TEX615
Protein detection: Lamin-B1
Cells: BT474 (breast cancer cells)
Detection fluorophore: TEX615